Our findings highlight the coupling of the STING signaling platform to mitochondria dynamics.ĬCCP DRP1 IFN-β Mitochondrial dynamics STING TBK1.Ĭopyright © 2017 Elsevier Inc. Human physiology and biochemistry are studied systematically in lectures while anatomy is stressed in laboratory experiences requiring extensive dissection. Importantly, the knockout of the crucial mitochondria fission regulator Drp1 restored the STING activity, indicating that CCCP down-modulates the STING pathway through DRP1-mediated mitochondria fragmentation. Effect of valinomycin (A), nigericin (B), and CCCP (C) on ApH, AY, and AIE+. Biochemical and molecular-physiological aspects of the nitric oxide. O O.5 I.O VALINoMYCIN (PM) NIGERICIN (PM) cccP(AM) Fig.
#What is cccp in biochem download
We found that CCCP impairs the interaction between STING and TBK1 and concomitantly triggers mitochondria fission. CCCP is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential. Download scientific diagram Effect of the CCCP protonophore (10 M) on the NADH. Here, we show that CCCP inhibits activation of STING and its downstream signaling molecules, TBK1 and IRF3, but not STING translocation to the perinuclear region. However, how MMP dissipation leads to the suppression of the STING pathway remains unknown. Previous study showed that carbonyl cyanide 3-chlorophenylhydrazone (CCCP), the protonophore, inhibited STING-mediated IFN-β production via disrupting mitochondrial membrane potential (MMP). Carbonyl cyanide-m-chlorophenylhydrazone (CCCP) decreased the minimum inhibitory concentration (MIC) of TMP-SXT by eight to 128 folds in all 14 isolates. Recently, multiple mechanisms regulating the activity of the STING pathway have been revealed. The uncoupler of oxidative phosphorylation blocks ATP. Besides its important role in innate immune response to DNA virus infection, the regulatory function of STING in autoimmunity and cancer is emerging. CCCP (carbonyl cyanide m-chlorophenyl hydrazine) is widely used uncoupler of oxidative phosphorylation.